For identification of macromolecular complexes in cryoelectron tomo- grams covering cytoplasmic regions, we used a combination of subtomogram averaging and classification. In a first step, the cross correlation function between two times binned tomograms and a 25 nm spherical template was computed. From the corresponding correlation peaks subvolumes (24 × 24 × 24 voxels) were extracted and classified into 12 classes via a missing-wedge corrected multi reference classification procedure, using the initial template with different representations of Gaussian noise added as starting references.


1 Answer 1


I found the answer myself :)

In transmission electron microscopy one obtains a 3D image by tilting the sample stage. Usually it's 180°-x and x is called "missing wedge".

Look here: https://en.wikipedia.org/wiki/Transmission_electron_microscopy#Three-dimensional_imaging


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