I calculated SASA for 4 systems using the following command. The trajectory file (run.xtc) is not treated with gmx trjconv option. In my MD simulation, I followed two annealing cycles in each 50 ns simulation time. In the graph, I observed a pattern at every 25 ns and I am unable to explain why I get it. I would like to know whether I did any incorrect step in calculating SASA or whether this SASA behavior is normal in annealing. Appreciate your help on this.

gmx sasa -s run.tpr -f .run.xtc -o sasa_run.xvg -n index.ndx -tu ns

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